CD23 Recombinant Rabbit Monoclonal Antibody [PD00-03]

About this product

CD23 (low affinity IgE receptor, Leu-20, FceRII) is a type II integral membrane glycoprotein, 45-60 kDa, and a member of an immunoglobulin supergene family. CD23 is a B-cell-specific antigen and it has essential roles in the regulation of IgE production and in the differentiation of B-cells. In humans, main cellular expression of CD23 is found in B-lymphocytes (strong expression in activated germinal center B-cells, weaker staining of resting mantle zone B-cells), monocytes, follicular dendritic cells (FDCs) predominately in the apical light zone of the germinal center, but also on activated CD4+ T lymphocyte subset, platelets, eosinophils, neutrophils, and Langerhans cells. CD23 is upregulated in Eppstein-Barr infection. CD23 is constitutively expressed by intestinal epithelial cells and its expression is enhanced in enteropathies. CD23 is typically expressed in chronic lymphocytic leukemia (CLL), sometimes in follicular lymphoma, rarely in marginal zone and lymphoplasmacytic lymphoma, but not in mantle cell lymphoma. In CLL, the strongest expression is characteristically present in proliferation centers. It was recently demonstrated that patients with CD23-positive diffuse large B-cell lymphoma have good prognosis. CD23 is also frequently used to demonstrate benign follicular dendritic cells in the background of follicular lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, (intra)follicular T-cell lymphoma and other lymphomas. Together with CD21, CD23 is a marker of rare follicular dendritic cell tumors Some authors demonstrated CD23 expression in epithelial cells of nasopharyngeal carcinoma. In diagnostic pathology CD23 is primarily used in the panel for small B-cell lymphoproliferative disorders. Tonsil is recommended as positive and negative tissue control for CD23. The follicular dendritic cells of the germinal centres must be stained as strongly as possible without any staining reaction of squamous epithelial cells and T-cells in the interfollicular T-zones. In the mantle zone of the follicles, the majority of activated B-cells must show an at least weak to moderate and distinct continuous membranous staining reaction.

Specifications

Catalog number

HA721139

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Blast 2 antibody BLAST-2 antibody Blast2 antibody C type lectin domain family 4 member J antibody C-type lectin domain family 4 antibody C-type lectin domain family 4 member J antibody C-type lectin domain family 4, member J antibody CD 23 antibody CD 23A antibody CD23 antibody CD23 antigen antibody CD23A antibody CLEC 4J antibody CLEC4J antibody Fc epsilon receptor II antibody Fc epsilon RII antibody Fc fragment of IgE antibody Fc fragment of IgE low affinity II receptor for antibody Fc fragment of IgE receptor II antibody Fc fragment of IgE, low affinity II, receptor for (CD23) antibody Fc of IgE antibody Fc of IgE, low affinity II, receptor for (CD23) antibody Fc receptor IgE low affinity II alpha polypeptide antibody Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody Fc-epsilon-RII antibody FCE 2 antibody FCE2 antibody FCER 2 antibody Fcer2 antibody FCER2_HUMAN antibody FCER2A antibody FceRII antibody IgE binding factor antibody IgE receptor lymphocyte antibody IgE-binding factor antibody IGEBF antibody Immunoglobulin E binding factor antibody Immunoglobulin E receptor antibody Immunoglobulin E receptor, low affinity II antibody Immunoglobulin E-binding factor antibody Immunoglobulin epsilon chain antibody LEUKOCYTE ANTIGEN CD23 antibody Low Affinity IgE Receptor antibody Low affinity immunoglobulin epsilon Fc receptor antibody Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody Low affinity immunoglobulin epsilon Fc receptor soluble form antibody Ly-42 antibody Ly42 antibody Lymphocyte antigen CD23 antibody Lymphocyte IgE receptor antibody MGC93219 antibody

Abbreviation

FCER2

Swiss Prot

P06734 Human

Cellular Locus

Cell membrane, Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within human CD23 aa 48 – 321 (Extracellular).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IHC-P, mIHC

Positive Control

Human tonsil tissue, human lymph nodes tissue, human spleen tissue.

Concentration

1 mg/mL

Dilution

IHC-P: 1:200-1:2,000 ;mIHC: 1:800

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 36 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes