Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18]

Catalog number HA721144

Discontinued

Price for HA721144

Size / count 100 µL

This variant is discontinued and cannot be added to your cart.

About this product

Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine. Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis . Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity.

Specifications

Catalog number: HA721144
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: Yes
CAS Number: 9000-83-3
Product Name Alternative: 1810036L03Rik antibody DF 5L antibody DF5L antibody DFNA 5L antibody DFNA5L antibody FKSG 10 antibody FKSG10 antibody FLJ12150 antibody Gasdermin antibody Gasdermin D antibody Gasdermin domain containing 1 antibody Gasdermin domain containing protein 1 antibody Gasdermin domain-containing protein 1 antibody Gasdermin-D antibody GasderminD antibody GSDMD antibody GSDMD_HUMAN antibody GSDMDC 1 antibody GSDMDC1 antibody
Abbreviation: GSDMD
Swiss Prot: P57764 Human, Q9D8T2 Mouse,
Cellular Locus: Cell membrane, Secreted.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein within Gasdermin D full length protein.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IHC-P, IF-Cell
Positive Control: SiHa cell lysate, Jurkat cell lysate, THP-1 cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, L6 cell lysate, THP-1 cell lysate treated with PMA for 17.5 hours then treated with LPS for 6 hours, mouse intestine tissue.
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:2,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 53/30 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18]?

The catalog number for Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18] is HA721144.

Is Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18] in stock?

Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Gasdermin D (N terminal) Recombinant Rabbit Monoclonal Antibody [PD00-18] used for?

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.