SOX2 Recombinant Rabbit Monoclonal Antibody [PO00-28]

About this product

The differentiation of seminomas from non-seminomatous germ cell tumors can be challenging, especially, if small biopsy specimens, necrotic tumors and metastatic tumors with artifacts are encountered. A subset of germ cell tumors may require immunohistochemistry (IHC) for classification owing to unusual morphologic features, such as diffuse growth of clear cells, and tumors with glandular and/or microcytic patterns.1 In the mixed germ cell tumor, one component is often intermingled intimately with others such as embryonal carcinoma versus yolk sac tumor, can be overlooked. IHC will identify such an area and allow for the identification of each component of the mixed tumor more accurately and documenting them in the pathology report is recommended by WHO. Current IHC studies have shown the combination of CD30/CD117 staining plays a good role in distinguishing between embryonal carcinoma and yolk sac tumor. However, a subset of tumors may not be distinguished by this combination. Also, the characteristic membranous pattern by antibodies to CD30 and CD117 for the interpretation of the diagnosis may not be evident in limited biopsy specimens. In this respect, transcription factors, such as SOX-2, are easier to interpret due to their distinct nuclear reaction. SOX-2 has been reported as a diagnostic marker for embryonal carcinoma. SOX-2 was expressed in intratubular embryonal carcinoma, pure embryonal carcinoma and in the embryonal carcinoma component of mixed germ cell tumor in all cases. But, SOX-2 expression has not been found in seminoma, yolk sac tumor, and choriocarcinoma in almost all cases.

Specifications

Catalog number

HA721155

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ANOP3 antibody cb236 antibody Delta EF2a antibody lcc antibody MCOPS3 antibody MGC148683 antibody MGC2413 antibody RGD1565646 antibody Sex determining region Y box 2 antibody SOX 2 antibody Sox2 antibody SOX2_HUMAN antibody SRY (sex determining region Y) box 2 antibody SRY box containing gene 2 antibody SRY related HMG box 2 antibody SRY related HMG box gene 2 antibody SRY-box 2 antibody Transcription factor SOX 2 antibody Transcription factor SOX-2 antibody ysb antibody

Abbreviation

SOX2

Swiss Prot

P48431 Human, P48432 Mouse,

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human SOX2 aa 1-317.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IHC-P, WB, IF-Cell, ChIP, IHC-Fr, IF-Tissue

Positive Control

NCCIT cell lysate, F9 cell lysate, NCCIT, F9, human trachea tissue, human cervical carcinoma tissue, human glioma tissue, human esophagus tissue, human tonsil tissue, human lung cancer tissue, mouse brain tissue, mouse lung tissue, rat brain tissue, rat hippocampus tissue, E14.5 mouse embryonic brain tissue.

Concentration

1 mg/mL

Dilution

IHC-P: 1:1,000-1:4,000 ;WB: 1:1,000 ;IF-Cell: 1:100-1:500 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin. ;IHC-Fr: 1:1,000 ;IF-Tissue: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 34 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes