Androgen receptor Recombinant Rabbit Monoclonal Antibody [PO00-29]

About this product

Androgen receptor (AR) is a member of the steroid receptor superfamily that is essential for the growth of prostate cancer cells. As a member of the steroid hormone superfamily, androgen receptor functions within distinct intracellular compartments to mediate cellular growth and transcriptional activation of androgen-regulated signaling pathways. This is accomplished through interactions between steroid ligand and androgen receptor molecules in the cytoplasm that promote dimerization and conformational change. The ligand-receptor homodimers are subsequently translocated to the nucleus for binding to genetic response elements and enhancer regions. Androgen receptor expression is broadly distributed across a variety of normal tissue types as well as in carcinomas of the breast and urothelium, and specifically plays a critical role in prostate development, homeostasis, and carcinogenesis. It has been reported that tyrosine phosphorylation of AR is induced by growth factors and elevated in hormone-refractory prostate tumors. Data suggest that growth factors and their downstream tyrosine kinases, which are elevated during hormone-ablation therapy, can induce tyrosine phosphorylation of AR. Such modification may be important for prostate tumor growth under androgen-depleted conditions. Cellular signaling occurs following androgen binding to the AR and translocation to the nucleus. This activated complex associates with androgen-responsive elements contained in the DNA sequence of target genes, affecting the transcriptional activity of these genes. AR antibody labels epithelial cells and stromal cells in normal prostate. AR reactivity is also found in other types of cells, including epithelial cells of the breast and hepatocytes. In prostate cancer, AR expression is maintained throughout cancer progression. Immunohistochemistry of AR is useful for the evaluation of prostate cancer in routinely processed tissues. The majority of androgen independent hormone refractory prostate cancers express AR. Transcriptional activation of AR is involved in refractory antiandrogen therapy. Androgen receptor expression has been helpful in predicting the clinical response to anti-androgenic treatment.

Specifications

Catalog number

HA721156

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

Yes

CAS Number

9000-83-3

Product Name Alternative

AIS antibody ANDR_HUMAN antibody Androgen nuclear receptor variant 2 antibody Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody Androgen receptor antibody androgen receptor splice variant 4b antibody AR antibody AR8 antibody DHTR antibody Dihydro testosterone receptor antibody Dihydrotestosterone receptor (DHTR) antibody Dihydrotestosterone receptor antibody HUMARA antibody HYSP1 antibody KD antibody Kennedy disease (KD) antibody NR3C4 antibody Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody Nuclear receptor subfamily 3 group C member 4 antibody SBMA antibody SMAX1 antibody Spinal and bulbar muscular atrophy (SBMA) antibody Spinal and bulbar muscular atrophy antibody Testicular Feminization (TFM) antibody TFM antibody

Abbreviation

AR

Swiss Prot

P10275 Human, P19091 Mouse, P15207 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Androgen Receptor aa 300-400 (N terminal).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Tissue, IF-Cell

Positive Control

A549 cell lysate, K-562 cell lysate, 22RV1 cell lysate, rat testis tissue lysates, 22RV1, human prostate carcinoma tissue, human fallopian tube tissue, mouse testis tissue, mouse epididymis tissue, rat epididymis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:1,000 ;IF-Tissue: 1:50-1:200 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 99 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes