MSH6 Recombinant Rabbit Monoclonal Antibody [PD00-26]

About this product

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. Tonsil is found to be a recommendable positive tissue control for MSH6. Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining reaction, while a moderate to strong nuclear staining reaction must be seen in the proliferating germinal centre B-cells. Colon adenocarcinoma with loss of MSH6 expression is recommended as negative tissue control. No nuclear staining reaction should be seen in the neoplastic cells, whereas a nuclear staining reaction must be seen in stromal cells serving as internal positive tissue control.

Specifications

Catalog number

HA721164

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DNA mismatch repair protein Msh6 antibody G/T mismatch binding protein antibody G/T mismatch-binding protein antibody GTBP antibody GTMBP antibody hMSH6 antibody HNPCC 5 antibody HNPCC5 antibody HSAP antibody MSH 6 antibody MSH6 antibody MSH6_HUMAN antibody mutS (E. coli) homolog 6 antibody MutS alpha 160 kDa subunit antibody MutS homolog 6 (E. coli) antibody mutS homolog 6 antibody MutS-alpha 160 kDa subunit antibody p160 antibody Sperm associated protein antibody

Abbreviation

MSH6

Swiss Prot

P52701 Human, P54276 Mouse

Cellular Locus

Nucleus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human MSH6 aa 350-450 (internal sequence).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, IF-Tissue

Positive Control

A549 cell lysate, HepG2 cell lysate, human tonsil tissue, human colon carcinoma tissue, human stomach carcinoma tissue, mouse small intestine tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 153 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes