SQSTM1 / p62 Recombinant Rabbit Monoclonal Antibody [PS00-61]

About this product

Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR (KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes.

Specifications

Catalog number

HA721171

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

A170 antibody DMRV antibody EBI 3 associated protein of 60 kDa antibody EBI 3 associated protein p60 antibody EBI3 associated protein of 60 kDa antibody EBI3 associated protein p60 antibody EBI3-associated protein of 60 kDa antibody EBIAP antibody FTDALS3 antibody MGC127197 antibody ORCA antibody OSF-6 antibody Osi antibody OSIL antibody Oxidative stress induced like antibody p60 antibody p62 antibody p62B antibody Paget disease of bone 3 antibody PDB 3 antibody PDB3 antibody Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody PKC-zeta-interacting protein antibody Protein kinase C-zeta-interacting protein antibody Sequestosome 1 antibody Sequestosome-1 antibody SQSTM 1 antibody SQSTM_HUMAN antibody Sqstm1 antibody STAP antibody STONE14 antibody Ubiquitin binding protein p62 antibody Ubiquitin-binding protein p62 antibody ZIP 3 antibody ZIP antibody ZIP3 antibody

Abbreviation

Sequestosome-1 EBI3-associated protein of 60 kDa (EBIAP; p60) Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 SQSTM1 ORCA OSIL

Swiss Prot

Q13501 Human, Q64337 Mouse, O08623 Rat

Cellular Locus

Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human SQSTM1/ p62 aa 400 to the C-terminus

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, FC, IF-Tissue, IHC-Fr

Positive Control

HeLa cell lysate, HeLa treated with 50μM Chloroquine for 18 hours cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, SH-SY5Y cell lysate, PANC-1 cell lysate, C2C12, PC-12, A549, human colon carcinoma tissue, human liver tissue, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000-1:10,000 ;IHC-P: 1:1,000-1:2,000 ;IF-Cell: 1:100-1:1,000 ;FC: 1:1,000 ;IF-Tissue: 1:200-1:1,000 ;IHC-Fr: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 48 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes