Mesothelin Recombinant Rabbit Monoclonal Antibody [PD00-68]

About this product

Mesothelin is a 40 kDa protein that is expressed in mesothelial cells. The protein was first identified by its reactivity with monoclonal antibody K1. Subsequent cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known. A knockout mouse line that lacks mesothelin reproduces and develops normally. Mesothelin is over expressed in several human tumors, including mesothelioma, ovarian cancer, pancreatic adenocarcinoma, lung adenocarcinoma, and cholangiocarcinoma. Mesothelin binds MUC16 (also known as CA125), indicating that the interaction of mesothelin and MUC16 may contribute to the implantation and peritoneal spread of tumors by cell adhesion. The region (residues 296-359) consisting of 64 amino acids at the N-terminus of cell surface mesothelin has been identified as the functional binding domain (named IAB) for MUC16/CA125, suggesting the mechanism of mesothelin acting as a MUC16/CA125 functional partner in cancer development.

Specifications

Catalog number

HA721175

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

CAK 1 antibody CAK1 antibody CAK1 antigen antibody cleaved form antibody Megakaryocyte potentiating factor antibody Mesothelin antibody Mesothelin isoform 1 precursor antibody MPF antibody Msln antibody MSLN_HUMAN antibody Pre pro megakaryocyte potentiating factor antibody Pre-pro-megakaryocyte-potentiating factor antibody SMR antibody SMRP antibody Soluble MPF mesothelin related protein antibody

Abbreviation

MSLN

Swiss Prot

Q13421 Human

Cellular Locus

Cell membrane, Golgi apparatus; Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide within Human Mesothelin aa 550 to the C-terminus.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

HeLa cell lysate, SK-OV-3 cell lysate, OVCAR-3 cell lysate, SiHa cell lysate, NCI-H226 cell lysate, human tonsil tissue, human ovarian carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200-1:800

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 69 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes