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Product details

HRP Conjugated Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29]

Catalog number HA721186

Price for HA721186

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Size / count 100 µL

About this product

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Specifications

Catalog number
HA721186
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9000-83-3
Product Name Alternative
a actin antibody ACTA antibody ACTA1 antibody Actin alpha skeletal muscle antibody Actin antibody actin, alpha 1, skeletal muscle 1 antibody actin, alpha 1, skeletal muscle antibody Actin, alpha skeletal muscle antibody actina antibody actine antibody ACTS_HUMAN antibody aktin antibody Alpha Actin 1 antibody Alpha skeletal muscle Actin antibody alpha skeletal muscle antibody alpha-actin antibody Alpha-actin-1 antibody ASMA antibody CFTD antibody CFTD1 antibody CFTDM antibody MPFD antibody NEM1 antibody NEM2 antibody NEM3 antibody nemaline myopathy type 3 antibody
Abbreviation
ACTA1
Swiss Prot
P68133 Human, P68134 Mouse, P68136 Rat
Cellular Locus
Cytoplasm, Cytoskeleton.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within Human Actin aa 45-80 / 377.
Isotype
IgG
Conjugation
HRP-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB
Positive Control
Hela cell lysate, A431 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate.
Concentration
1 mg/mL
Dilution
WB: 1:5,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
Molecular Weight
Predicted band size: 42 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes

Frequently Asked Questions

What is the catalog number for HRP Conjugated Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29]?

The catalog number for HRP Conjugated Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29] is HA721186.

What sizes are available for HRP Conjugated Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29]?

HRP Conjugated Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29] is available in the following size(s): 100 µL.

Is HRP Conjugated Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29] in stock?

HRP Conjugated Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29] is currently made to order / not held in immediate stock. Contact us for lead time.

What is HRP Conjugated Actin Recombinant Rabbit Monoclonal Antibody [JJ09-29] used for?

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only on

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

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