IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89]

Catalog number HA721205

Price for HA721205

Size / count 100 µL

About this product

A J chain is a protein component of the antibodies IgM and IgA. It is a 137 residue polypeptide, encoded by the IGJ gene. The J Chain is required for IgM or IgA to be secreted into mucosa. As part of a polymeric immunoglobulin (pIg), the J-chain is essential for binding of pIg to the pIgR, which forms the secretory component upon excretion of the secretory pIg by epithelial cells. This binding facilitates transport of the J-chain positive pIg molecules from the basal to the apical sides of epithelial cells. Because IgM and IgA are the only two types of antibody that polymerize, initial hypotheses stated that J chain was required for polymerization. However, it was subsequently found that IgM is able to polymerize in the absence of J chain as both a pentamer and a hexamer, however, both of these exist to lesser numbers in organisms lacking J chains. In such cases, there are also fewer IgA dimers. The J-chain also plays a role in the activation of complement. J-chain negative IgM hexamers are 15-20 times more effective at activating complement than J-chain positive IgM pentamers. A consequence of this lack of complement activation is it allows J-chain positive pIgM to bind antigens without causing excessive damage to epithelial membranes through complement activation.

Specifications

Catalog number: HA721205
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: IGCJ antibody IGJ_HUMAN antibody Immunoglobulin J chain antibody Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides antibody immunoglobulin joining chain antibody JCH antibody JCHAIN antibody Joining chain of multimeric IgA and IgM antibody
Abbreviation: JCHAIN, IGCJ, IGJ
Swiss Prot: P01591 Human
Cellular Locus: Secreted.
Host: Rabbit
Species Reactivity: Human
Immunogen: Synthetic peptide within Human IGJ aa 50-150 (internal sequence).
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: IHC-P, FC
Positive Control: Human tonsil tissue, Daudi.
Concentration: 1 mg/mL
Dilution: IHC-P: 1:2,000 ;FC: 1:500-1:1,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 18 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89]?

The catalog number for IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89] is HA721205.

What sizes are available for IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89]?

IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89] is available in the following size(s): 100 µL.

Is IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89] in stock?

IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89] is currently made to order / not held in immediate stock. Contact us for lead time.

What is IGJ Recombinant Rabbit Monoclonal Antibody [PD00-89] used for?

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.