Nucleophosmin Recombinant Rabbit Monoclonal Antibody [PD00-91]

About this product

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. NPM1 gene is up-regulated, mutated and chromosomally translocated in many tumor types. Chromosomal aberrations involving NPM1 were found in patients with non-Hodgkin lymphoma, acute promyelocytic leukemia, myelodysplastic syndrome, and acute myelogenous leukemia. Heterozygous mice for NPM1 are vulnerable to tumor development. In solid tumors NPM1 is frequently found overexpressed, and it is thought that NPM1 could promote tumor growth by inactivation of the tumor suppressor p53/ARF pathway; on the contrary, when expressed at low levels, NPM1 could suppress tumor growth by the inhibition of centrosome duplication. Of high importance is NPM involvement in acute myelogenous leukemia, where a mutated protein lacking a folded C-terminal domain (NPM1c+) has been found in the cytoplasm in patients. This aberrant localization has been linked to the development of the disease, and is associated with improved clinical outcomes. Strategies against this subtype of acute myelogenous leukemia include the refolding of the C-terminal domain using pharmalogical chaperones and the displacement of the protein from nucleolus to nucleoplasm, which has been linked to apoptotic mechanisms. It has also been shown that in the context of clonal hematopoiesis of undetermined significance harboring a DNMT3A mutation, subsequent NPM1 mutations drive progression into overt myeloproliferative neoplasm.

Specifications

Catalog number

HA721206

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

B23 antibody MGC104254 antibody NO38 antibody NPM antibody NPM_HUMAN antibody NPM1 antibody Nucleolar phosphoprotein B23 antibody Nucleolar protein NO38 antibody Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody Nucleophosmin antibody nucleophosmin nucleoplasmin family member 1 antibody Nucleophosmin/nucleoplasmin family member 1 antibody Numatrin antibody OTTHUMP00000161024 antibody OTTHUMP00000161025 antibody OTTHUMP00000223397 antibody OTTHUMP00000223398 antibody

Abbreviation

NPM1, NPM

Swiss Prot

P06748 Human, Q61937 Mouse, P13084 Rat

Cellular Locus

Nucleus, nucleolus, nucleoplasm, cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Nucleophosmin aa 1-100 (N terminal).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, HeLa, rat kidney tissue, mouse small intestine tissue, human colon carcinoma tissue, human skin tissue, Daudi, HL-60.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:5,000 ;IHC-P: 1:200-1:500 ;FC: 1:500-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 33 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes