p40 Recombinant Rabbit Monoclonal Antibody [PDH0-02]

About this product

p63 protein (p63) is a nuclear protein, a transcription factor. The p40 is an isoform of p63, a transcription factor that regulates many cell activities, including cell proliferation, maintenance, and differentiation. It performs as a sensitive and specific tool for aiding in the identification of squamous cell carcinoma of the lung. In addition to its utility as a squamous differentiation marker, p40 has also been proven to be a valuable marker for highlighting myoepithelial and basal cell populations in prostate, breast, skin, and salivary gland. Strong p40 expression is frequently observed in esophageal cancerous squamous lesions. Immunohistochemical detection of p40 can also be helpful in identifying urothelial carcinoma. In cases of prostate carcinoma, p40 is almost always found to be negative for basal cell staining. Among carcinomas, p40 has approximately the same sensitivity as p63 but a higher specificity, as the TAp63 isoform is expressed more widespread in eg., adenocarcinomas. Moreover, p63 occur in lymphomas that are p40 negative. Placenta is recommended as positive tissue control for p40, where an at least weak to moderate, distinct nuclear staining reaction of cytotrophoblasts must be seen. The cytotrophoblasts should be visible even at low magnification (5x objective). Supportive to placenta, tonsil can be used as positive and negative tissue control. Virtually all squamous epithelial cells must show a moderate to strong, distinct nuclear staining reaction. No nuclear or cytoplasmic staining reaction should be seen in other cell types.

Specifications

Catalog number

HA721222

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

40 kDa Rab9 effector protein antibody 8430412M01Rik antibody 9530020d24rik antibody AV073337 antibody bA65N13.1 antibody C87311 antibody DKFZp686P1077 antibody OTTHUMP00000022126 antibody OTTMUSP00000012898 antibody OTTMUSP00000012899 antibody OTTMUSP00000041318 antibody RAB9 effector p40 antibody Rab9 effector protein with Kelch motifs antibody RAB9p40 antibody RABEK_HUMAN antibody RABEPK antibody RABEPK protein antibody RGD1310612 antibody RP11 65N13.1 antibody RP23-446N16.2 antibody

Swiss Prot

Q9H3D4 Human

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human p40 aa 2-50 / 586 (Q9H3D4-2)

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IHC-P, WB, IF-Cell

Positive Control

Human tonsil tissue, human skin tissue, human lung squamous cell carcinoma tissue, human bladder carcinoma tissue, human prostate tissue, human breast tissue, rat skin tissue, mouse bladder tissue, A431 cell lysates, A431.

Concentration

1 mg/mL

Dilution

IHC-P: 1:1,000-1:2,000 ;WB: 1:1,000 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 77 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes