Smad4 Recombinant Rabbit Monoclonal Antibody [PD01-37]

About this product

The SMAD4 gene is located on chromosome 18q21 and encodes the SMAD4 protein belonging to the SMAD family of transcription factor proteins, which act as mediators of TGF-β signal transduction. The TGF-β/SMAD4 signaling pathway controls signal transduction from cell membrane to nucleus, and is responsible for a wide range of cellular processes such as proliferation, differentiation, apoptosis, migration, as well as cancer initiation and progression. Alterations in the SMAD4 gene was primarily discovered in pancreatic cancer (duct adenocarcinoma) but occur in a variety of cancers such as colorectal cancer, gastric cancer, prostate cancer, melanomas, head and neck cancers and many others, though with higher frequencies in gastrointestinal tract cancers. Loss of SMAD4 expression in tumors has also been shown to affect cancer progression and therapy, such as reduced response to adjuvant chemotherapy. Tonsil is recommendable as external control for SMAD4: The vast majority of basal squamous epithelial cells in the surface epithelium must show a strong, predominantly nuclear staining reaction, whereas superficial squamous epithelial cells typically should be negative or only faintly demonstrated. Virtually all other cells e.g. lymphocytes must show an at least moderate, predominantly nuclear but also cytoplasmic staining reaction. In addition, a tumor (pancreas or colon) with loss of SMAD4 due to inactivation of the gene could be included, in which stromal cells (internal tissue control) should display the required staining reaction - at least moderate intensity. However, for SMAD4 it has to be emphasized that internal positive tissue controls being e.g. normal stromal cells adjacent to the neoplastic cells are preferred to external controls. An observed intact nuclear expression of SMAD4 proteins in the internal normal cells together with loss of SMAD4 proteins in the neoplastic cells is diagnostic essential.

Specifications

Catalog number

HA721234

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody Deleted in Pancreatic Carcinoma antibody Deleted in pancreatic carcinoma locus 4 antibody Deletion target in pancreatic carcinoma 4 antibody DPC 4 antibody DPC4 antibody hSMAD4 antibody JIP antibody MAD homolog 4 antibody MAD mothers against decapentaplegic Drosophila homolog 4 antibody MAD mothers against decapentaplegic homolog 4 antibody MADH 4 antibody MADH4 antibody Med antibody Medea antibody Mothers against decapentaplegic homolog 4 antibody Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody Mothers against DPP homolog 4 antibody MYHRS antibody OTTHUMP00000163548 antibody SMA- and MAD-related protein 4 antibody SMAD 4 antibody SMAD family member 4 antibody SMAD mothers against DPP homolog 4 antibody SMAD4 antibody SMAD4_HUMAN antibody

Abbreviation

MAD homolog 4, Mothers against DPP homolog 4, SMAD 4, Smad4, hSMAD4, SMAD4

Swiss Prot

Q13485 Human, P97471 Mouse, O70437 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Smad4 aa 500 to the C-terminus (C terminal).

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

HepG2 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, PANC-1, human lung carcinoma tissue, human testis tissue, human stomach carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 60 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes