SN38 Recombinant Rabbit Monoclonal Antibody [PSH0-06]

Catalog number HA721259

Price for HA721259

Size / count 100 µL

About this product

SN-38 is an antineoplastic drug. It is the active metabolite of irinotecan (an analog of camptothecin - a topoisomerase I inhibitor) but has 1000 times more activity than irinotecan itself. In vitro cytotoxicity assays show that the potency of SN-38 relative to irinotecan varies from 2- to 2000-fold. SN38 is formed via hydrolysis of irinotecan by carboxylesterases and metabolized via glucuronidation by UGT1A1. The variant of UGT1A1 in ~10% of Caucasians which leads to poor metabolism of SN-38 predicts irinotecan toxicity, as it is then less easily excreted from the body in its SN-38 glucuronide form. SN-38 and its glucuronide are lost into the bile and intestines. It can cause the symptoms of diarrhoea and myelosuppression experienced by ~25% of the patients administered irinotecan. SN-38 causes the strongest inhibition of DNA topoisomerase I, followed by CPT and then CPT-11. CPT-11 dose dependently shifts the position of relaxed DNA in the direction of nicked DNA, but SN-38 and CPT shows no effect on the position of relaxed DNA. SN-38 dose-dependently and time-dependently inhibit DNA synthesis. Respective IC50 values of SN-38, in DNA synthesis is 0.077 μM. The inhibitory effect of SN-38 on RNA synthesis is less than that on DNA synthesis and it does not inhibit protein synthesis. SN-38 caused frequent DNA single-strand breaks in P388 cells.

Specifications

Catalog number: HA721259
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: SN-38 antibody SN38 antibody
Abbreviation: SN-38 SN38
Host: Rabbit
Species Reactivity: Universal
Immunogen: SN38-OVA
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: ELISA, WB
Concentration: 1mg/mL
Dilution: ELISA: 1:10,000 ;WB: 1:1,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for SN38 Recombinant Rabbit Monoclonal Antibody [PSH0-06]?

The catalog number for SN38 Recombinant Rabbit Monoclonal Antibody [PSH0-06] is HA721259.

What sizes are available for SN38 Recombinant Rabbit Monoclonal Antibody [PSH0-06]?

SN38 Recombinant Rabbit Monoclonal Antibody [PSH0-06] is available in the following size(s): 100 µL.

Is SN38 Recombinant Rabbit Monoclonal Antibody [PSH0-06] in stock?

SN38 Recombinant Rabbit Monoclonal Antibody [PSH0-06] is currently made to order / not held in immediate stock. Contact us for lead time.

What is SN38 Recombinant Rabbit Monoclonal Antibody [PSH0-06] used for?

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.