ISG15 Recombinant Rabbit Monoclonal Antibody [JE32-27]

About this product

Interferon-stimulated gene 15 (ISG15) is a 17 kDA secreted protein that in humans is encoded by the ISG15 gene. ISG15 is induced by type I interferon (IFN) and serves many functions, acting both as an extracellular cytokine and an intracellular protein modifier. The precise functions are diverse and vary among species but include potentiation of Interferon gamma (IFN-II) production in lymphocytes, ubiquitin-like conjugation to newly-synthesized proteins and negative regulation of the IFN-I response. ISG15-deficiency is a very rare genetic disorder caused by mutations of the ISG15 gene. It is inherited with an autosomal recessive pattern and is classified as a primary immunodeficiency or inborn error of immunity. Patients present in childhood with infectious, neurologic or dermatologic features. Basal ganglia calcification is observed in all patients reported to date and represents the underlying autoinflammatory disease of excessive IFN-I activity, known as type I interferonopathy. The basal ganglia calcifications may cause epileptic seizures but often are asymptomatic. The IFN-I inflammation may also manifest early in life as ulcerative skin lesions in the armpit, groin and neck regions. Finally, ISG15-deficiency leads to mendelian susceptibility to mycobacterial disease, although with incomplete penetrance. These infections present as fistulizing lymphadenopathies and respiratory symptoms following BCG vaccination. In pancreatic ductal adenocarcinoma, tumor-associated macrophages secrete ISG15 enhancing the phenotype of cancer stem cells in the tumor.

Specifications

Catalog number

HA721264

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

G1P2 antibody hUCRP antibody IFI 15 antibody IFI15 antibody Interferon alpha inducible protein antibody Interferon induced protein 15 antibody Interferon induced protein IFI-15K antibody Interferon stimulated protein 15 kDa antibody Interferon, alpha-inducible protein (clone IFI-15K) antibody Interferon-induced 15 kDa protein antibody Interferon-induced 17 kDa protein antibody Interferon-induced 17-kDa/15-kDa protein antibody IP17 antibody ISG 15 antibody ISG15 antibody ISG15 ubiquitin like modifier antibody ISG15_HUMAN antibody Ubiquitin cross-reactive protein antibody Ubiquitin-like protein ISG15 antibody UCRP antibody

Abbreviation

ISG15, G1P2, UCRP

Swiss Prot

P05161 Human

Cellular Locus

Cytoplasm, Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant full length protein of Human ISG15.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

Hela treated with 10 ng/ml human IFN alpha 1 for 16 hours whole cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 18 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes