HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76]

Catalog number M1005-3

Price for M1005-3

Size / count 100 µL

About this product

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Specifications

Catalog number: M1005-3
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Cellular Locus: serum plasma
Host: Mouse
Species Reactivity: Human
Immunogen: Purified Human IgG heavy chain.
Isotype: IgG2a
Conjugation: HRP-conjugated
Type: Mouse monoclonal Antibody
Applications: WB, ELISA
Positive Control: Human serum lysates.
Concentration: 2 mg/mL
Dilution: WB: 1:5,000 ;ELISA: 1:5,000
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
Molecular Weight: Predicted band size: 50 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76]?

The catalog number for HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76] is M1005-3.

What sizes are available for HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76]?

HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76] is available in the following size(s): 100 µL.

Is HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76] in stock?

HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76] is currently made to order / not held in immediate stock. Contact us for lead time.

What is HRP Conjugated Mouse anti-Human IgG heavy chain Mouse Monoclonal Antibody [54-76] used for?

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.