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Product details

HRP Conjugated Mouse anti-Human IgG light chain (kappa chain) Mouse Monoclonal Antibody [A11-G11]

Catalog number M1007-11

Price for M1007-11

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Size / count 100 µL

About this product

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Specifications

Catalog number
M1007-11
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Cellular Locus
Serum/ Plasma
Host
Mouse
Species Reactivity
Human
Immunogen
Human IgG light chain
Isotype
IgG2a
Conjugation
HRP-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, ELISA
Positive Control
Human IgG
Concentration
2 mg/mL
Dilution
WB: 1:2,000-1:5,000 ;ELISA: 1:1,000
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
Molecular Weight
25 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No

Frequently Asked Questions

What is the catalog number for HRP Conjugated Mouse anti-Human IgG light chain (kappa chain) Mouse Monoclonal Antibody [A11-G11]?

The catalog number for HRP Conjugated Mouse anti-Human IgG light chain (kappa chain) Mouse Monoclonal Antibody [A11-G11] is M1007-11.

What sizes are available for HRP Conjugated Mouse anti-Human IgG light chain (kappa chain) Mouse Monoclonal Antibody [A11-G11]?

HRP Conjugated Mouse anti-Human IgG light chain (kappa chain) Mouse Monoclonal Antibody [A11-G11] is available in the following size(s): 100 µL.

Is HRP Conjugated Mouse anti-Human IgG light chain (kappa chain) Mouse Monoclonal Antibody [A11-G11] in stock?

HRP Conjugated Mouse anti-Human IgG light chain (kappa chain) Mouse Monoclonal Antibody [A11-G11] is currently made to order / not held in immediate stock. Contact us for lead time.

What is HRP Conjugated Mouse anti-Human IgG light chain (kappa chain) Mouse Monoclonal Antibody [A11-G11] used for?

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The who

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

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