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Product details

HRP Conjugated Human lambda light chain Mouse Monoclonal Antibody [36-59]

Catalog number M1007-12

Price for M1007-12

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Size / count 100 µL

About this product

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Specifications

Catalog number
M1007-12
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Cellular Locus
Serum/ Plasma
Host
Mouse
Species Reactivity
Human
Immunogen
Human lambda light chain.
Isotype
IgG2a
Conjugation
HRP-conjugated
Type
Mouse monoclonal Antibody
Applications
WB, ELISA
Positive Control
Human serum lysates.
Concentration
2 mg/mL
Dilution
WB: 1:500-1:1,000 ;ELISA: 1:500-1:1,000
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
Molecular Weight
Predicted band size: 25 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No

Frequently Asked Questions

What is the catalog number for HRP Conjugated Human lambda light chain Mouse Monoclonal Antibody [36-59]?

The catalog number for HRP Conjugated Human lambda light chain Mouse Monoclonal Antibody [36-59] is M1007-12.

What sizes are available for HRP Conjugated Human lambda light chain Mouse Monoclonal Antibody [36-59]?

HRP Conjugated Human lambda light chain Mouse Monoclonal Antibody [36-59] is available in the following size(s): 100 µL.

Is HRP Conjugated Human lambda light chain Mouse Monoclonal Antibody [36-59] in stock?

HRP Conjugated Human lambda light chain Mouse Monoclonal Antibody [36-59] is currently made to order / not held in immediate stock. Contact us for lead time.

What is HRP Conjugated Human lambda light chain Mouse Monoclonal Antibody [36-59] used for?

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The who

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

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