DNA-PKcs/PRKDC Mouse Monoclonal Antibody [2-B3]

About this product

DNA-dependent protein kinase, catalytic subunit, also known as DNA-PKcs, is an enzyme that in humans is encoded by the gene designated as PRKDC or XRCC7. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. The DNA-Pkcs protein is a serine/threonine protein kinase comprising a single polypeptide chain of 4,128 amino acids. DNA-PKcs is the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase called DNA-PK. The second component is the autoimmune antigen Ku. On its own, DNA-PKcs is inactive and relies on Ku to direct it to DNA ends and trigger its kinase activity. DNA-PKcs is required for the non-homologous end joining (NHEJ) pathway of DNA repair, which rejoins double-strand breaks. It is also required for V (D)J recombination, a process that utilizes NHEJ to promote immune system diversity. DNA-PKcs knockout mice have severe combined immunodeficiency due to their V (D)J recombination defect. Many proteins have been identified as substrates for the kinase activity of DNA-PK. Autophosphorylation of DNA-PKcs appears to play a key role in NHEJ and is thought to induce a conformational change that allows end processing enzymes to access the ends of the double-strand break.[9] DNA-PK also cooperates with ATR and ATM to phosphorylate proteins involved in the DNA damage checkpoint.

Specifications

Catalog number

M1204-6

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

DNA dependent protein kinase catalytic subunit antibody DNA PK catalytic subunit antibody DNA-dependent protein kinase catalytic subunit antibody DNA-PK catalytic subunit antibody DNA-PKcs antibody DNAPK antibody DNAPK catalytic subunit antibody DNPK 1 antibody DNPK1 antibody Hyper radiosensitivity of murine scid mutation, complementing 1 antibody Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody HYRC 1 antibody HYRC antibody HYRC1 antibody IMD26 antibody p350 antibody p460 antibody PKRDC antibody PRKDC antibody PRKDC_HUMAN antibody Protein Kinase DNA Activated Catalytic Polypeptide antibody XRCC 7 antibody XRCC7 antibody

Abbreviation

DNA-PK catalytic subunit,DNA-PKcs

Swiss Prot

P78527 Human, P97313 Mouse

Cellular Locus

Nucleus, nucleolus.

Host

Mouse

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein within Mouse DNA-PKcs/PRKDC aa 3,900-4,128/4,128.

Isotype

IgG2a

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

K-562 cell lysate, HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, 293T cell lysate, HT-29 cell lysate, A549 cell lysate, MCF7 cell lysate, HepG2, human breast carcinoma tissue, human prostate carcinoma tissue, human colon cancer tissue.

Concentration

2 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:500

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 469 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No