beta Actin Mouse Monoclonal Antibody [B4-B2] — image 1 of 3

Product details

beta Actin Mouse Monoclonal Antibody [B4-B2]

Catalog number M1210-2

Price for M1210-2

Size / count 100 µL

About this product

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

Specifications

Catalog number: M1210-2
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: A26C1A antibody A26C1B antibody ACTB antibody ACTB_HUMAN antibody Actin beta antibody Actin cytoplasmic 1 antibody Actin, cytoplasmic 1, N-terminally processed antibody Actx antibody b actin antibody Beta cytoskeletal actin antibody Beta-actin antibody BRWS1 antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody
Abbreviation: ACTB
Swiss Prot: P60709 Human, P60710 Mouse, P60711 Rat
Cellular Locus: Cytoplasm.
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within N-terminal of Human beta actin.
Isotype: IgG1
Conjugation: Non-conjugated
Type: Mouse monoclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, A431 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, mouse spleen tissue lysate, rat testis tissue lysate, rat kidney tissue lysate, NIH/3T3, human colon carcinoma tissue, mouse kidney tissue.
Concentration: 2 mg/mL
Dilution: WB: 1:20,000 ;IF-Cell: 1:100-1:200 ;IHC-P: 1:100-1:200 ;FC: 1:100-1:200
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 42 kDa
Storage Conditions: Store at -20°C. Stable for 12 months from date of receipt.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for beta Actin Mouse Monoclonal Antibody [B4-B2]?

The catalog number for beta Actin Mouse Monoclonal Antibody [B4-B2] is M1210-2.

What sizes are available for beta Actin Mouse Monoclonal Antibody [B4-B2]?

beta Actin Mouse Monoclonal Antibody [B4-B2] is available in the following size(s): 100 µL.

Is beta Actin Mouse Monoclonal Antibody [B4-B2] in stock?

beta Actin Mouse Monoclonal Antibody [B4-B2] is currently made to order / not held in immediate stock. Contact us for lead time.

What is beta Actin Mouse Monoclonal Antibody [B4-B2] used for?

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.