EEA1 Mouse Monoclonal Antibody [B0-A8] — image 1 of 3

Product details

EEA1 Mouse Monoclonal Antibody [B0-A8]

Catalog number M1503-1

Price for M1503-1

Size / count 100 µL

About this product

EEA1 localizes exclusively to early endosomes?and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate?through its C-terminalFYVE domain?and forms a homodimer?through a?coiled coil. EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as?N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. Due to the proteins importance in vesicular trafficking, a number of intracellular bacteria prevent EEA1 recruitment to the vacuole.Mycobacterium tuberculosis?is known to inhibit the recruitment of EEA1 to the phagosomal membrane through CamKII. Legionella pneumophila?also prevents EEA1 recruitment through a currently unknown mechanism.[5] Interestingly, the related pathogen Legionella longbeachae?recruits EEA1 and appears to replicate within a modified early endosome.

Specifications

Catalog number: M1503-1
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: Early endosome antigen 1 antibody Early endosome antigen 1, 162kD antibody Early endosome associated protein antibody EEA 1 antibody EEA1 antibody EEA1_HUMAN antibody Endosome associated protein p162 antibody Endosome-associated protein p162 antibody MST105 antibody MSTP105 antibody ZFYVE2 antibody Zinc finger FYVE domain containing protein 2 antibody Zinc finger FYVE domain-containing protein 2 antibody
Abbreviation: EEA1
Swiss Prot: Q15075 Human, Q8BL66 Mouse, A0A0G2K051 Rat
Cellular Locus: Cytoplasm, early endosome membrane.
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide of the N terminal residues of Human EEA1.
Isotype: IgG1
Conjugation: Non-conjugated
Type: Mouse monoclonal Antibody
Applications: WB, IHC-P
Positive Control: 293, human colon cancer tissue, mouse prostate tissue.
Concentration: 2 mg/mL
Dilution: WB: 1:2,000-1:10,000 ;IHC-P: 1:50-1:200
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 162 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for EEA1 Mouse Monoclonal Antibody [B0-A8]?

The catalog number for EEA1 Mouse Monoclonal Antibody [B0-A8] is M1503-1.

What sizes are available for EEA1 Mouse Monoclonal Antibody [B0-A8]?

EEA1 Mouse Monoclonal Antibody [B0-A8] is available in the following size(s): 100 µL.

Is EEA1 Mouse Monoclonal Antibody [B0-A8] in stock?

EEA1 Mouse Monoclonal Antibody [B0-A8] is currently made to order / not held in immediate stock. Contact us for lead time.

What is EEA1 Mouse Monoclonal Antibody [B0-A8] used for?

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.