GRP78 / BIP Mouse Monoclonal Antibody [2-7]

About this product

Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Like many stress and heat shock proteins, BiP/GRP78 has potent immunological activity when released from the internal environment of the cell into the extracelluar space.specifically, it feeds anti-inflammatory and pro-resolutory signals into immune networks, thus helping to resolve inflammation.

Specifications

Catalog number

M1505-13

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

78 kDa glucose regulated protein antibody 78 kDa glucose-regulated protein antibody AL022860 antibody AU019543 antibody BIP antibody D2Wsu141e antibody D2Wsu17e antibody Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78 antibody Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody Epididymis secretory sperm binding protein Li 89n antibody FLJ26106 antibody Glucose Regulated Protein 78kDa antibody GRP 78 antibody GRP-78 antibody GRP78 antibody GRP78_HUMAN antibody Heat shock 70 kDa protein 5 antibody Heat Shock 70kDa Protein 5 antibody Heat shock protein family A (Hsp70) member 5 antibody HEL S 89n antibody Hsce70 antibody HSPA 5 antibody HSPA5 antibody Immunoglobulin Heavy Chain Binding Protein antibody Immunoglobulin heavy chain-binding protein antibody mBiP antibody MIF2 antibody Sez7 antibody

Abbreviation

GRP-78,BiP

Swiss Prot

P11021 Human, P20029 Mouse, P06761 Rat

Cellular Locus

Cytoplasm, endoplasmic reticulum lumen

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Synthetic peptide within Human GRP78 aa 605-654 / 654.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, IF-Tissue, FC

Positive Control

L-929 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat pancreas tissue lysate, Hela, hybrid fish (crucian-carp) heart tissue lysates.

Concentration

2 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:250 ;IHC-P: 1:200-1,000 ;IF-Tissue: 1:50-1:200 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 78 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No