SIRT1 Mouse Monoclonal Antibody [7-C5-B2]

About this product

Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, SIRT1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors. In mammals, SIRT1 has been shown to deacetylate and thereby deactivate the p53 protein. SIRT1 also stimulates autophagy by preventing acetylation of proteins (via deacetylation) required for autophagy as demonstrated in cultured cells and embryonic and neonatal tissues. This function provides a link between sirtuin expression and the cellular response to limited nutrients due to caloric restriction. SIRT1 plays a role in activating T helper 17 cells, which contribute to autoimmune disease; efforts to activate SIRT1 therapeutically may trigger or exacerbate autoimmune disease. SIRT1, along with HDAC1 and the AP-1 promoter complex within D1-type dopaminergic medium spiny neurons, appears to be closely involved in the pathogenesis of addiction.

Specifications

Catalog number

M1506-3

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

75SirT1 antibody hSIR2 antibody hSIRT1 antibody HST2, S. cerevisiae, homolog of antibody NAD dependent deacetylase sirtuin 1 antibody NAD dependent protein deacetylase sirtuin 1 antibody OTTHUMP00000198111 antibody OTTHUMP00000198112 antibody Regulatory protein SIR2 homolog 1 antibody SIR1_HUMAN antibody SIR2 antibody SIR2 like 1 antibody SIR2 like protein 1 antibody SIR2, S.cerevisiae, homolog-like 1 antibody SIR2-like protein 1 antibody SIR2ALPHA antibody SIR2L1 antibody Sirt1 antibody SirtT1 75 kDa fragment antibody Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody Sirtuin 1 antibody Sirtuin type 1 antibody

Abbreviation

hSIRT1,hSIR2

Swiss Prot

Q96EB6 Human

Cellular Locus

Nucleus, PML body, Cytoplasm.

Host

Mouse

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human SIRT1 aa 698-747 / 747.

Isotype

IgG2b

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

Hela, human lung carcinoma tissue, human colon carcinoma tissue

Concentration

2 mg/mL

Dilution

IHC-P: 1:200 ;IF-Cell: 1:200-1:500 ;WB: 1:2000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 82 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No