PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [A3-D0]

About this product

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of (p85) and (p110) subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and γ, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.

Specifications

Catalog number

M1510-2

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

GRB1 antibody p85 alpha antibody p85 antibody P85A_HUMAN antibody Phosphatidylinositol 3 kinase associated p 85 alpha antibody Phosphatidylinositol 3 kinase regulatory 1 antibody Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody Phosphatidylinositol 3-kinase regulatory subunit alpha antibody Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody PI3 kinase p85 subunit alpha antibody PI3-kinase regulatory subunit alpha antibody PI3-kinase subunit p85-alpha antibody PI3K antibody PI3K regulatory subunit alpha antibody Pik3r1 antibody PtdIns 3 kinase p85 alpha antibody PtdIns-3-kinase regulatory subunit alpha antibody PtdIns-3-kinase regulatory subunit p85-alpha antibody

Abbreviation

PI3-kinase regulatory subunit alpha,PI3K regulatory subunit alpha,PtdIns-3-kinase regulatory subunit alpha,PI3-kinase subunit p85-alpha,PtdIns-3-kinase regulatory subunit p85-alpha

Swiss Prot

P27986 Human

Cellular Locus

Cytosol, Membrane, Nucleus.

Host

Mouse

Species Reactivity

Human

Immunogen

Recombinant protein within Human PI3-kinase p85 subunit alpha aa 19-219 / 724.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell

Positive Control

SW480 cell lysate, A431 cell lysate, 293T cell lysate, Jurkat cell lysate, A549 cell lysate.

Concentration

2 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 84 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No