Grp75 Mouse Monoclonal Antibody [A6-A5-B1]

About this product

The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity

Specifications

Catalog number

M1603-1

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

75 kDa glucose regulated protein antibody 75 kDa glucose-regulated protein antibody CSA antibody Glucose Regulated Protein antibody Grp 75 antibody GRP-75 antibody GRP75 antibody GRP75_HUMAN antibody Heat shock 70 kDa protein 9 antibody Heat shock 70kD protein 9 antibody heat shock 70kDa protein 9 antibody Heat shock 70kDa protein 9B antibody Heat shock protein 74 kDa A antibody Heat shock protein A antibody Heat shock protein cognate 74 antibody Hsc74 antibody Hsp74 antibody Hsp74a antibody HSPA9 antibody Hspa9a antibody HSPA9B antibody MGC4500 antibody mitochondrial antibody Mortalin 2 antibody Mortalin antibody Mortalin perinuclear antibody Mortalin2 antibody MOT 2 antibody MOT antibody MOT2 antibody Mthsp70 antibody p66 mortalin antibody P66 MOT antibody PBP74 antibody Peptide binding protein 74 antibody Peptide-binding protein 74 antibody Stress 70 protein mitochondrial antibody Stress 70 protein mitochondrial precursor antibody Stress-70 protein antibody

Abbreviation

GRP-75, MOT, PBP74

Swiss Prot

P38646 Human, P38647 Mouse, P48721 Rat

Cellular Locus

Mitochondrion, Nucleus

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Grp75 aa 630-679 / 679.

Isotype

IgM

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, FC

Positive Control

HeLa cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, HeLa, NIH/3T3, HepG2.

Concentration

2 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:100 ;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 74 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No