Tyrosinase Mouse Monoclonal Antibody [C2-B5]

About this product

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone. Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.

Specifications

Catalog number

M1701-2

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ATN antibody CMM8 antibody LB24 AB antibody LB24-AB antibody Monophenol monooxygenase antibody OCA1 antibody OCA1A antibody OCAIA antibody Oculocutaneous albinism IA antibody SHEP3 antibody SK29 AB antibody SK29-AB antibody Tumor rejection antigen AB antibody TYR antibody TYRO_HUMAN antibody tyrosinase (oculocutaneous albinism IA) antibody Tyrosinase antibody

Abbreviation

TYR

Swiss Prot

P14679 Human, Q2TA50 Mouse

Cellular Locus

Membrane.

Host

Mouse

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human Tyrosinase aa 51-100 / 529.

Isotype

IgG1

Conjugation

Non-conjugated

Type

Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Melanoma cells lysates, B16F1, human urethral melanoma tissue.

Concentration

2 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 60 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

No