Histone H3 (mono methyl K4) Rabbit Polyclonal Antibody

About this product

The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.

Specifications

Catalog number

R1110-1

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

HIST1 cluster, H3J antibody Histone gene cluster 1, H3 histone family, member E antibody Histone gene cluster 1, H3G antibody H3 histone family, member J antibody HIST1 cluster, H3E antibody HIST1 cluster, H3I antibody Histone gene cluster 1, H3C antibody FLJ92264 antibody H3 histone family, member A antibody H3 histone family, member B antibody H3 histone family, member C antibody H3 histone family, member D antibody H3 histone family, member F antibody H3 histone family, member H antibody H3 histone family, member I antibody H3 histone family, member K antibody H3 histone family, member L antibody H3 histone family, member T antibody H3 histone, family 3A antibody H3.1 antibody H3.3A antibody H3/a antibody H3/b antibody H3/c antibody H3/d antibody h3/f antibody H3/h antibody H3/i antibody H3/j antibody H3/k antibody H3/l antibody H3/t antibody H31_HUMAN antibody H3F1K antibody H3F3 antibody H3F3A antibody H3FA antibody H3FB antibody H3FC antibody H3FD antibody H3FF antibody H3FH antibody H3FI antibody H3FJ antibody H3FK antibody H3FL antibody HIST1 cluster, H3A antibody HIST1 cluster, H3B antibody HIST1 cluster, H3C antibody HIST1 cluster, H3D antibody HIST1 cluster, H3F antibody HIST1 cluster, H3G antibody HIST1 cluster, H3H antibody HIST1H3A antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody Histone 1, H3a antibody Histone 1, H3b antibody Histone 1, H3c antibody Histone 1, H3d antibody Histone 1, H3e antibody Histone 1, H3f antibody Histone 1, H3g antibody Histone 1, H3h antibody Histone 1, H3i antibody Histone 1, H3j antibody histone 3, H3 antibody histone cluster 1 H3 family member a antibody histone cluster 1 H3 family member b antibody histone cluster 1 H3 family member c antibody histone cluster 1 H3 family member d antibody histone cluster 1 H3 family member e antibody histone cluster 1 H3 family member f antibody histone cluster 1 H3 family member g antibody histone cluster 1 H3 family member h antibody histone cluster 1 H3 family member i antibody histone cluster 1 H3 family member j antibody Histone cluster 1, H3a antibody Histone cluster 1, H3b antibody Histone cluster 1, H3c antibody Histone cluster 1, H3d antibody Histone cluster 1, H3e antibody Histone cluster 1, H3f antibody Histone cluster 1, H3g antibody Histone cluster 1, H3i antibody Histone cluster 1, H3j antibody Histone gene cluster 1, H3 histone family, member A antibody Histone gene cluster 1, H3 histone family, member B antibody Histone gene cluster 1, H3 histone family, member C antibody Histone gene cluster 1, H3 histone family, member D antibody Histone gene cluster 1, H3 histone family, member F antibody Histone gene cluster 1, H3 histone family, member G antibody Histone gene cluster 1, H3 histone family, member H antibody Histone gene cluster 1, H3 histone family, member I antibody Histone gene cluster 1, H3 histone family, member J antibody Histone gene cluster 1, H3A antibody Histone gene cluster 1, H3B antibody Histone gene cluster 1, H3D antibody Histone gene cluster 1, H3E antibody Histone gene cluster 1, H3F antibody Histone gene cluster 1, H3H antibody Histone gene cluster 1, H3I antibody Histone gene cluster 1, H3J antibody Histone H 3 antibody Histone H3.1 antibody histone H3.1t antibody Histone H3.2 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody Histone H3/m antibody Histone H3/o antibody H3K4me1 antibody

Abbreviation

HIST1H3A H3K4me1

Swiss Prot

P68431 Human, P68433 Mouse, Q497B8 Rat

Cellular Locus

Nucleus

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human Histone H3 aa 1-49/136.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, Dot Blot

Positive Control

NCCIT, PC-12, MCF-7, human liver carcinoma tissue, human colon carcinoma tissue, human pancreas tissue, SHSY5Y.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;Dot Blot: 1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 15 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No