Cyclin B1 Rabbit Polyclonal Antibody

About this product

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis.

Specifications

Catalog number

R1308-13

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

CCNB 1 antibody CCNB antibody ccnb1 antibody CCNB1_HUMAN antibody Cyclin B1 antibody G2 mitotic specific cyclin B1 antibody G2/mitotic-specific cyclin-B1 antibody

Abbreviation

CCNB1

Swiss Prot

P14635 Human

Cellular Locus

Cytoplasm, nucleus, Cytoskeleton.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human Cyclin B1 aa 384-433 / 433.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

293 cell lysate, Hela, F9.

Concentration

1 mg/mL

Dilution

WB: 1:500;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:500;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 48 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No