CHFR Rabbit Polyclonal Antibody — image 1 of 2

Product details

CHFR Rabbit Polyclonal Antibody

Catalog number R1310-3

Price for R1310-3

Size / count 100 µL

About this product

E3 ubiquitin-protein ligase (CHFR) that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. CHFR is probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation. Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2'-deoxycytidine has been reported.

Specifications

Catalog number: R1310-3
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: Checkpoint with forkhead and ring finger domains antibody Checkpoint with forkhead and RING finger domains protein antibody Chfr antibody CHFR_HUMAN antibody E3 ubiquitin protein ligase CHFR antibody E3 ubiquitin-protein ligase CHFR antibody EC 6.3.2. antibody FLJ10796 antibody FLJ33629 antibody RING finger protein 196 antibody RNF 116 antibody RNF 196 antibody RNF116 antibody RNF196 antibody Ubiquitin ligase protein antibody
Abbreviation: CHFR
Swiss Prot: Q96EP1 Human
Cellular Locus: Nucleus
Host: Rabbit
Species Reactivity: Human
Immunogen: peptide
Isotype: IgG
Conjugation: Non-conjugated
Type: Rabbit polyclonal Antibody
Applications: WB, IF-Cell
Positive Control: Hela, A431
Concentration: 1 mg/mL
Dilution: WB: 1:500-1:1,000;IF-Cell: 1:200
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: ~73kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for CHFR Rabbit Polyclonal Antibody?

The catalog number for CHFR Rabbit Polyclonal Antibody is R1310-3.

What sizes are available for CHFR Rabbit Polyclonal Antibody?

CHFR Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is CHFR Rabbit Polyclonal Antibody in stock?

CHFR Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is CHFR Rabbit Polyclonal Antibody used for?

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.