EEA1 Rabbit Polyclonal Antibody — image 1 of 3

Product details

EEA1 Rabbit Polyclonal Antibody

Catalog number R1401-23

Price for R1401-23

Size / count 100 µL

About this product

EEA1 localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms a homodimer through a coiled coil . EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. Due to the proteins importance in vesicular trafficking, a number of intracellular bacteria prevent EEA1 recruitment to the vacuole. Mycobacterium tuberculosis is known to inhibit the recruitment of EEA1 to the phagosomal membrane through CamKII.

Specifications

Catalog number: R1401-23
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: Early endosome antigen 1 antibody Early endosome antigen 1, 162kD antibody Early endosome associated protein antibody EEA 1 antibody EEA1 antibody EEA1_HUMAN antibody Endosome associated protein p162 antibody Endosome-associated protein p162 antibody MST105 antibody MSTP105 antibody ZFYVE2 antibody Zinc finger FYVE domain containing protein 2 antibody Zinc finger FYVE domain-containing protein 2 antibody
Abbreviation: EEA1
Swiss Prot: Q15075 Human, Q8BL66 Mouse
Cellular Locus: Cytoplasm, early endosome membrane.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic peptide within N-terminal human EEA1.
Isotype: IgG
Conjugation: Non-conjugated
Type: Rabbit polyclonal Antibody
Applications: WB, IF-Cell, IHC-P, FC
Positive Control: NIH/3T3, Hela, Jurkat, 293T, A431, rat brain tissue, rat prostate tissue, mouse colon tissue.
Concentration: 1 mg/mL
Dilution: WB:1:1,000 ;IF-Cell:1:100-1:200 ;IHC-P:1:50-1:200 ;FC:1:50-1:100
Purity: Immunogen affinity purified.
Form: Liquid
Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: 162 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: No

Frequently Asked Questions

What is the catalog number for EEA1 Rabbit Polyclonal Antibody?

The catalog number for EEA1 Rabbit Polyclonal Antibody is R1401-23.

What sizes are available for EEA1 Rabbit Polyclonal Antibody?

EEA1 Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is EEA1 Rabbit Polyclonal Antibody in stock?

EEA1 Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is EEA1 Rabbit Polyclonal Antibody used for?

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.