STAT1 Rabbit Polyclonal Antibody

About this product

All STAT molecules are phosphorylated by receptor associated kinases, that causes activation, dimerization by forming homo- or heterodimers and finally translocate to nucleus to work as transcription factors. Specifically STAT1 can be activated by several ligands such as Interferon alpha (IFNα), Interferon gamma (IFNγ), Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Interleukin 6 (IL-6), or IL-27. Type I interferons (IFN-α, IFN-ß) bind to receptors, cause signaling via kinases, phosphorylate and activate the Jak kinases TYK2 and JAK1 and also STAT1 and STAT2. STAT molecules form dimers and bind to ISGF3G/IRF-9, which is Interferon stimulated gene factor 3 complex with Interferon regulatory Factor 9. This allows STAT1 to enter the nucleus.[6] STAT1 has a key role in many gene expressions that cause survival of the cell, viability or pathogen response. There are two possible transcripts (due to alternative splicing) that encode 2 isoforms of STAT1. STAT1α, the full-length version of the protein, is the main active isoform, responsible for most of the known functions of STAT1. STAT1ß, which lacks a portion of the C-terminus of the protein, is less-studied, but has variously been reported to negatively regulate activation of STAT1 or to mediate IFN-γ-dependent anti-tumor and anti-infection activities. STAT1 is involved in upregulating genes due to a signal by either type I, type II, or type III interferons. In response to IFN-γ stimulation, STAT1 forms homodimers or heterodimers with STAT3 that bind to the GAS (Interferon-Gamma-Activated Sequence) promoter element; in response to either IFN-α or IFN-β stimulation, STAT1 forms a heterodimer with STAT2 that can bind the ISRE (Interferon-Stimulated Response Element) promoter element. In either case, binding of the promoter element leads to an increased expression of ISG (Interferon-Stimulated Genes). Expression of STAT1 can be induced with diallyl disulfide, a compound in garlic.

Specifications

Catalog number

R1408-2

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Signal transducer and activator of transcription 1 91kD antibody CANDF7 antibody DKFZp686B04100 antibody IMD31A antibody IMD31B antibody IMD31C antibody ISGF 3 antibody ISGF-3 antibody OTTHUMP00000163552 antibody OTTHUMP00000165046 antibody OTTHUMP00000165047 antibody OTTHUMP00000205845 antibody Signal transducer and activator of transcription 1 91kDa antibody Signal transducer and activator of transcription 1 antibody Signal transducer and activator of transcription 1, 91kD antibody Signal transducer and activator of transcription 1-alpha/beta antibody STAT 1 antibody Stat1 antibody STAT1_HUMAN antibody STAT91 antibody Transcription factor ISGF 3 components p91 p84 antibody Transcription factor ISGF-3 components p91/p84 antibody

Swiss Prot

P42224 Human, P42225 Mouse,

Cellular Locus

Cytoplasm, nucleus

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Recombinant protein within human STAT1 aa 87-262.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell

Positive Control

Hela, 293, A431, NIH/3T3

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:200

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 87 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No