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Product details

Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody

Catalog number R1501-3

Price for R1501-3

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Size / count 100 µL

About this product

The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.

Specifications

Catalog number
R1501-3
Size / count
100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Abbreviation
R.FokI
Swiss Prot
P14870 Planomicrobium okeanokoites
Host
Rabbit
Species Reactivity
Planomicrobium okeanokoites
Immunogen
Synthetic peptide within C-terminal of Planomicrobium okeanokoites fokIR
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB
Positive Control
Purified restriction enzyme FokI
Concentration
1 mg/mL
Dilution
WB: 1:1,000-1:2,000
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 66 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No

Frequently Asked Questions

What is the catalog number for Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody?

The catalog number for Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody is R1501-3.

What sizes are available for Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody?

Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody is available in the following size(s): 100 µL.

Is Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody in stock?

Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody used for?

The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGAT

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

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