MLCK Rabbit Polyclonal Antibody

About this product

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and myosin light chain kinases (MYLK, also designated MLCK). The MYLK kinases phosphorylate myosin regulatory light chains to catalyze myosin interaction with actin filaments resulting in contractile activity. Non-muscle, smooth muscle and skeletal/cardiac muscle MYLK isoforms exist. The MYLK gene (also designated MYLK1) encodes both smooth muscle and non-muscle isoforms as well as telokin, a small C-terminal isoform expressed only in smooth muscle with the capacity to stabilize unphosphorylated myosin filaments. Multiple transcript variants are described for the MYLK gene. Smooth-muscle and non-muscle MYLK isoforms are expressed in a wide variety of adult and fetal tissues. The skeletal/cardiac muscle isoforms of MYLK are encoded by a separate gene, MYLK2 (also designated skMLCK). MYLK appears to be a target for PAKs (p21-activated kinases). PAK1 interaction with MYLK results in a decrease in MYLK activity and myosin light chain phosphorylation.

Specifications

Catalog number

R1510-18

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

deglutamylated form antibody DKFZp686I10125 antibody EC 2.7.11.18 antibody FLJ12216 antibody Kinase related protein antibody Kinase-related protein antibody KRP antibody MLCK antibody MLCK1 antibody MLCK108 antibody MLCK210 antibody MSTP083 antibody MYLK antibody MYLK_HUMAN antibody MYLK1 antibody Myosin light chain kinase antibody Myosin light polypeptide kinase antibody OTTHUMP00000180642 antibody OTTHUMP00000180643 antibody smMLCK antibody smooth muscle antibody Smooth muscle myosin light chain kinase antibody Telokin antibody

Abbreviation

MLCK,smMLCK,KRP

Swiss Prot

Q15746 Human

Cellular Locus

Cytoplasm, Cleavage furrow, Cell projection

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human MLCK aa 36-85 / 1,914.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

IF-Cell, IHC-P, FC

Positive Control

Hela, NIH/3T3, SHG-44, mouse smooth muscle tissue.

Concentration

1 mg/mL

Dilution

IF-Cell: 1:50-1:200;IF-Cell: 1:50-1:200;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No