PHD2 Rabbit Polyclonal Antibody

About this product

Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-α subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-α proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3. HIF PHD2 is regarded as the main cellular oxygen sensor, as RNA interference against HIF PHD2, but not HIF PHD1 or HIF PHD3, is enough to stabilize HIF-1α in normoxia. HIF PHD2, a direct HIF target gene, is expressed mainly in skeletal muscle, heart, kidney and brain.

Specifications

Catalog number

R1510-40

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

C1ORF12 antibody Chromosome 1 Open Reading Frame 12 antibody DKFZp761F179 antibody ECYT 3 antibody ECYT3 antibody EGL 9 homolog of C. elegans 1 antibody EGL nine (C.elegans) homolog 1 antibody Egl nine homolog 1 (C. elegans) antibody Egl nine homolog 1 antibody Egl nine like protein 1 antibody EGLN 1 antibody Egln1 antibody EGLN1_HUMAN antibody HIF PH2 antibody HIF Prolyl Hydroxylase 2 antibody HIF-PH2 antibody HIF-prolyl hydroxylase 2 antibody HIFP4H 2 antibody HIFPH2 antibody HPH 2 antibody HPH-2 antibody HPH2 antibody Hypoxia inducible factor prolyl hydroxylase 2 antibody Hypoxia-inducible factor prolyl hydroxylase 2 antibody ORF13 antibody P4H2 antibody PHD 2 antibody PhD2 antibody PNAS 118 antibody PNAS 137 antibody Prolyl Hydroxylase Domain Containing Protein 2 antibody Prolyl hydroxylase domain-containing protein 2 antibody Rat Homolog of SM20 antibody SM 20 antibody SM-20 antibody SM20 antibody Zinc finger MYND domain containing protein 6 antibody ZMYND6 antibody

Abbreviation

HIF-PH2,HIF-prolyl hydroxylase 2,HPH-2,PHD2

Swiss Prot

Q9GZT9 Human, Q91YE3 Mouse, P59722 Rat

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human PHD2 aa 377-426 / 426.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

PC-12, human kidney tissue, mouse kidney tissue, human pancreas tissue, mouse brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:10-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 46kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No