Ku80 Rabbit Polyclonal Antibody

About this product

Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

Specifications

Catalog number

R1511-17

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

86 kDa subunit of Ku antigen antibody ATP dependent DNA helicase 2 subunit 2 antibody ATP dependent DNA helicase II 80 kDa subunit antibody ATP dependent DNA helicase II 86 Kd subunit antibody ATP dependent DNA helicase II antibody ATP-dependent DNA helicase 2 subunit 2 antibody ATP-dependent DNA helicase II 80 kDa subunit antibody CTC box binding factor 85 kDa antibody CTC box-binding factor 85 kDa subunit antibody CTC85 antibody CTCBF antibody DNA repair protein XRCC5 antibody KARP 1 antibody KARP1 antibody Ku 80 antibody Ku autoantigen 80kDa antibody Ku80 antibody Ku86 antibody Ku86 autoantigen related protein 1 antibody KUB 2 antibody KUB2 antibody Lupus Ku autoantigen protein p86 antibody NFIV antibody Nuclear factor IV antibody Thyroid lupus autoantigen antibody Thyroid-lupus autoantigen antibody TLAA antibody X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody X-ray repair cross-complementing protein 5 antibody Xray repair complementing defective repair in Chinese hamster cells 5 antibody XRCC 5 antibody XRCC5 antibody XRCC5_HUMAN antibody

Abbreviation

CTC85,CTCBF,TLAA

Swiss Prot

P13010 Human

Cellular Locus

Nucleus. Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within human Ku80 aa 51-104.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

A549, Hela, MCF-7, human liver tissue lysate, human colon cancer tissue, human tonsil tissue, human breast cancer tissue, human kidney tissue.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2.000 ;IHC-P: 1:500-1:5,000 ;IF-Cell: 1:500-1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 83 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No