MYLK Rabbit Polyclonal Antibody

About this product

Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene. This gene, a muscle member of the immunoglobulin superfamily, encodes a myosin light-chain kinase, which is a calcium-/calmodulin-dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

Specifications

Catalog number

R1511-26

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

caMLCK antibody cardiac MLCK antibody Cardiac MyBP C associated Ca/CaM kinase antibody Cardiac-MyBP-C-associated Ca/CaM kinase antibody MLC kinase antibody MLCK antibody MLCK2 antibody Mylk3 antibody MYLK3_HUMAN antibody Myosin light chain kinase 3 antibody Putative myosin light chain kinase 3 antibody

Abbreviation

MLCK, smMLCK, KRP

Swiss Prot

Q15746 Human, Q6PDN3 Mouse

Cellular Locus

Cytoplasm, Cell projection, Cleavage furrow

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant protein with Human MYLK aa 230-470 / 1,914.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

IF-Cell, IHC-P, FC, WB

Positive Control

MCF-7, SW480, mouse colon tissue, mouse smooth muscle tissue, human kidney tissue lysates.

Concentration

1 mg/mL

Dilution

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:10-1:100 ;WB: 1:1,000

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 211 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No