Myosin Light Chain 2 Rabbit Polyclonal Antibody

About this product

Encoded by the MYL2 gene, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, also designated MLC-2 or MLC2v, is part of a hexameric complex of two heavy chains and four light chains predominantly expressed in adult cardiac ventricle muscle. Myosin regulatory light chain 2 binds calcium and has been shown to be a useful molecular marker for cardiac chamber specification. The co-expression of myosin regulatory light chain 7 (MYL7) and myosin regulatory light chain 2 in the outflow tract and atrioventricular canal, together with the single expression in the atrial (MYL7) and ventricular (MYL2) myocardium, permits the delineation of their boundaries. At the amino acid level there is 96% homology between the human and mouse myosin regulatory light chain sequences. Mutations in MYL2 are correlated with mid-left ventricular chamber type hypertrophic cardiomyopathy (MVC2) as well as familial hypertrophic cardiomyopathy type 10 (CMH10).

Specifications

Catalog number

R1512-19

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Cardiac myosin light chain-2 antibody Cardiac ventricular myosin light chain 2 antibody CMH10 antibody MLC 2v antibody MLC-2 antibody MLC-2v antibody MLC2 antibody MLRV_HUMAN antibody MYL 2 antibody MYL2 antibody Myosin light chain 2 regulatory cardiac slow antibody Myosin light polypeptide 2 regulatory cardiac slow antibody Myosin regulatory light chain 2 antibody Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody Regulatory light chain of myosin antibody RLC of myosin antibody Slow cardiac myosin regulatory light chain 2 antibody ventricular/cardiac muscle isoform antibody

Abbreviation

MLC-2, MLC-2v, MLC-2s/v, MYL2, MLC2

Swiss Prot

P10916 Human, P51667 Mouse, P08733 Rat

Cellular Locus

Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human Myosin Light Chain 2 aa 21-70 / 166.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

A431 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse skeletal tissue lysate, rat heart tissue lysate, A549, C2C12, mouse heart tissue, rat heart tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Immunogen affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 19 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No