IKK beta Rabbit Polyclonal Antibody

About this product

The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates Iκβ-α on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

Specifications

Catalog number

R1706-13

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

I kappa B kinase 2 antibody I kappa B kinase beta antibody I-kappa-B kinase 2 antibody I-kappa-B-kinase beta antibody IkBKB antibody IKK beta antibody IKK-B antibody IKK-beta antibody IKK2 antibody IKKB antibody IKKB_HUMAN antibody IMD15 antibody Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody Inhibitor of nuclear factor kappa-B kinase subunit beta antibody NFKBIKB antibody Nuclear factor NF-kappa-B inhibitor kinase beta antibody

Abbreviation

I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, IKK2, NFKBIKB, IKKB

Swiss Prot

O14920 Human, O88351 Mouse, Q9QY78 Rat

Cellular Locus

Nucleus. Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human IKK beta aa 501-649 / 756.

Isotype

IgG

Conjugation

Non-conjugated

Type

Rabbit polyclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

HL-60 cell lysate, THP-1 cell lysate, Hela, HUVEC, rat testis tissue, human tonsil tissue, human breast carcinoma tissue, human placenta tissue, mouse testis tissue, A549.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 87 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

No