Anti-ERK1/2 Antibody [SA43-03]

About this product

The first mitogen-activated protein kinase to be discovered was ERK1 (MAPK3) in mammals. Since ERK1 and its close relative ERK2 (MAPK1) are both involved in growth factor signaling, the family was termed "mitogen-activated". With the discovery of other members, even from distant organisms (e.g. plants), it has become increasingly clear that the name is a misnomer, since most MAPKs are actually involved in the response to potentially harmful, abiotic stress stimuli (hyperosmosis, oxidative stress, DNA damage, low osmolarity, infection, etc.). Because plants cannot "flee" from stress, terrestrial plants have the highest number of MAPK genes per organism ever found[citation needed]. Thus the role of mammalian ERK1/2 kinases as regulators of cell proliferation is not a generic, but a highly specialized function.

Specifications

Catalog number

ET1601-29TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ERK 1 antibody ERK 2 antibody ERK-2 antibody ERK1 antibody erk1/2 antibody ERK2 antibody ERT1 antibody ERT2 antibody Extracellular signal regulated kinase 1 antibody Extracellular signal-regulated kinase 2 antibody MAP kinase 1 antibody MAP kinase 2 antibody MAP kinase isoform p42 antibody MAP kinase isoform p44 antibody MAPK 1 antibody MAPK 2 antibody MAPK 3 antibody Mapk1 antibody MAPK2 antibody MAPK3 antibody Mitogen-activated protein kinase 1 antibody Mitogen-activated protein kinase 2 antibody MK01_HUMAN antibody p38 antibody p40 antibody p41 antibody p42-MAPK antibody PRKM 2 antibody

Abbreviation

MAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAP kinase 1, MAPK 1, ERK-2, p42-MAPK

Swiss Prot

P27361 Human; P28482 Human; P63085 Mouse; Q63844 Mouse; P21708 Rat; P63086 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Zebrafish

Immunogen

Recombinant protein within human ERK2 aa 180-379.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IP, FC, IHC-P, IHC-Fr

Positive Control

HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Jurkat, human breast carcinoma tissue, mouse esophagus tissue, mouse stomach tissue, Hela, MCF-7, NIH/3T3, A549.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:2,000 ;IF-Tissue: 1:50 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration. ;IHC-P: 1:20,000 ;IHC-Fr:1:50

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 43/41 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True