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Product details

Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09]

Catalog number ET1602-2TR

Price for ET1602-2TR

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Size / count 20 µL

About this product

Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. An important phosphorylated form is γH2AX (S139), which forms when double-strand breaks appear. In humans and other eukaryotes, the DNA is wrapped around histone octamers, consisting of core histones H2A, H2B, H3 and H4, to form chromatin. H2AX contributes to nucleosome-formation, chromatin-remodeling and DNA repair, and is also used in vitro as an assay for double-strand breaks in dsDNA.

Specifications

Catalog number
ET1602-2TR
Size / count
20 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Product Name Alternative
AW228881 antibody H2A histone family member X antibody H2A.FX antibody H2A.X antibody H2a/x antibody H2AFX antibody H2AX antibody H2AX histone antibody H2AX_HUMAN antibody Hist5.2ax antibody Histone 2A antibody Histone 2AX antibody Histone H2A.X antibody Histone H2AX antibody RGD1566119 antibody gamma H2A.X antibody γ H2A.X antibody γ-H2A.X antibody
Abbreviation
H2a/x, gamma H2A.X, H2AX,γ H2A.X,γ-H2A.X
Swiss Prot
P16104 Human; P27661 Mouse
Cellular Locus
Nucleus, Chromosome
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P
Positive Control
HeLa treated with 20μM Etoposide for 2 hours whole cell lysate, HeLa treated with UV for 2 hours whole cell lysate, NIH/3T3 treated with 25μM Etoposide for 5 hours whole cell lysate, HepG2 cell lysate–treated with etoposide, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse small intestine tissue, human colon carcinoma tissue.
Concentration
1 mg/mL
Dilution
WB: 1:2,000 ;IHC-P: 1:50-1:500
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 15 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
True

Frequently Asked Questions

What is the catalog number for Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09]?

The catalog number for Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09] is ET1602-2TR.

What sizes are available for Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09]?

Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09] is available in the following size(s): 20 µL.

Is Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09] in stock?

Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Anti-Phospho-Histone H2A.X (S139) Antibody [SR33-09] used for?

Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. An important phosphorylated form is γH2AX (S139), which forms when double-strand breaks appear. In humans and other eukaryotes, the DNA is wrapped around histone octamers, consisting of c

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

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