Anti-Phospho-Akt (Ser473) Antibody [SY28-05]

About this product

RAC (Rho family)-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) protein domains. It is commonly referred to as PKB, or by both names as "Akt/PKB". The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Specifications

Catalog number

ET1607-73TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

AKT 1 antibody AKT antibody AKT1 antibody AKT1_HUMAN antibody MGC99656 antibody PKB antibody PKB-ALPHA antibody PRKBA antibody Protein Kinase B Alpha antibody Protein kinase B antibody Proto-oncogene c-Akt antibody RAC Alpha antibody RAC antibody RAC-alpha serine/threonine-protein kinase antibody RAC-PK-alpha antibody

Abbreviation

PKB, PKB alpha, AKT1

Swiss Prot

P31749 Human; P31751 Human; Q9Y243 Human; P31750 Mouse

Cellular Locus

Cytoplasm, Nucleus, Cell membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser473 of human Akt1.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IHC-Fr

Positive Control

MCF7 treated with 100nM Calyculin A for 30 minutes whole cell lysate, NIH/3T3 treated with 100ng/mL PDGF for 1 hour whole cell lysate, C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate, mouse lung tissue, hela cell lysates, NIH/3T3 treated with 100nM Calyculin A for 30minutes whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30minutes and 10ng/mL hPDGF for 10minutes whole cell lysate, NIH/3T3.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IHC-Fr: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 56 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True