Anti-Cytokeratin 8 Antibody [SU0338]

About this product

Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. It is often paired with keratin 18. Antibodies to CK8 can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast. In normal tissue, it reacts mainly with secretory epithelia, but not with squamous epithelium, such as that found in the skin, cervix, and esophagus. However, it also reacts with a range of malignant cells, including those derived from secretory epithelia, but also some squamous carcinomata, such as spindle cell carcinoma. It is considered useful in identifying microscopic metastases of breast carcinoma in lymph nodes, and in distinguishing Paget's disease from malignant melanoma. It also reacts with neuroendocrine tumors. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

Specifications

Catalog number

ET1608-32TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

CARD2 antibody Cell proliferation inducing gene 46 protein antibody Cell proliferation inducing protein 46 antibody CK 8 antibody CK-8 antibody CK8 antibody CYK8 antibody Cytokeratin 8 antibody Cytokeratin-8 antibody K2C8 antibody K2C8_HUMAN antibody K8 antibody Keratin 8 antibody Keratin antibody keratin type II cytoskeletal 8 antibody Keratin-8 antibody KRT8 antibody type II cytoskeletal 8 antibody Type-II keratin Kb8 antibody

Abbreviation

CK-8, K8, KRT8, CYK8

Swiss Prot

P05787 Human; P11679 Mouse

Cellular Locus

Nucleoplasm, Nucleus matrix, Cytoplasm.

Host

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

Hela cell lysate, A431 cell lysate, Hela, MCF-7, A431, human liver tissue, human breast carcinoma tissue, human breast tissue, SK-Br-3, HepG2.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IF-Cell: 1:400-1:800 ;IF-Tissue: 1:400-1:800 ;IHC-P: 1:50-1:1,500 ;FC: 1:500-1:1,000 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 54 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

True