Anti-PARP Antibody [SU03-68]

About this product

The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. Once PARP detects a SSB, it binds to the DNA, undergoes a structural change, and begins the synthesis of a polymeric adenosine diphosphate ribose (poly (ADP-ribose) or PAR) chain, which acts as a signal for the other DNA-repairing enzymes. Target enzymes include DNA ligase III (LigIII), DNA polymerase beta (polβ), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly (ADP-ribose) glycohydrolase (PARG). PARP enzymes are essential in a number of cellular functions, including expression of inflammatory genes: PARP1 is required for the induction of ICAM-1 gene expression by cardiac myocytes and smooth muscle cells, in response to TNF.

Specifications

Catalog number

ET1608-56TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD (+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD (+) ADP ribosyltransferase 1 antibody NAD (+) ADP-ribosyltransferase 1 antibody pADPRT 1 antibody pADPRT1 antibody PARP 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly (ADP ribose) polymerase 1 antibody poly (ADP ribose) polymerase family, member 1 antibody Poly [ADP-ribose] polymerase 1 antibody Poly (ADP ribose) polymerase antibody poly (ADP ribose) synthetase antibody poly (ADP ribosyl)transferase antibody Poly[ADP ribose] synthetase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody

Abbreviation

PARP-1, ARTD1, ADPRT 1, PARP1

Swiss Prot

P09874 Human; P11103 Mouse; P27008 Rat

Cellular Locus

Nucleus, nucleolus, chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within N-terminal human PARP.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Positive Control

A549 cell lysate, Jurkat cell lysate, Hela cell lysate, HeLa, human spleen tissue, mouse large intestine tissue, human breast carcinoma tissue, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:500 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 113 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True