Anti-Caspase-1 Antibody [SU40-07]

About this product

Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1β and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias.

Specifications

Catalog number

ET1608-69TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Caspase1 CASP-1 antibody CASP1 antibody CASP1_HUMAN antibody Caspase1 antibody Caspase 1 antibody Caspase-1 subunit p10 antibody ICE antibody IL-1 beta-converting enzyme antibody IL-1BC antibody IL1 beta converting enzyme antibody IL1B convertase antibody Interleukin 1 beta convertase antibody Interleukin 1B converting enzyme antibody Interleukin-1 beta convertase antibody Interleukin-1 beta-converting enzyme antibody p45 antibody

Abbreviation

CASP-1, IL-1BC, ICE, IL-1 beta-converting enzyme, CASP1

Swiss Prot

P29466 Human; P29452 Mouse; P43527 Rat

Cellular Locus

Cytoplasm, Cell membrane, Membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human Caspase-1 aa 213-404 / 404.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, FC, IF-Cell

Positive Control

THP-1 cell lysate, THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours cell lysate, HCT 116 cell lysate, HL-60 cell lysate, RAW264.7 cell lysate, U937 cell lysates, mouse heart tissue lysate, rat heart tissue lysate, Hela, HL-60, THP-1.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:10,000 ;FC: 1:50-1:100 ;IF-Cell: 1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 45 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True