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Product details

Anti-Phospho-IKB alpha (S32) Antibody [ST53-05]

Catalog number ET1609-78TR

Price for ET1609-78TR

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Size / count 20 µL

About this product

On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to κB sites, suggesting that the IκB-α family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IκB family is represented by a protein designated IκB-β. The third group of IκB proteins is represented by IκB-γ, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional IκB family member, IκB-ε, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.

Specifications

Catalog number
ET1609-78TR
Size / count
20 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Product Name Alternative
I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
Abbreviation
IkB-alpha, IkappaBalpha, NFKBIA, IKBA, MAD3, NFKBI
Swiss Prot
P25963 Human; Q9Z1E3 Mouse
Cellular Locus
Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser32 of human IKB alpha.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IP
Positive Control
SK-Br-3 cell lysates, Hela,NIH-3T3 cell lysates.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
36 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
True

Frequently Asked Questions

What is the catalog number for Anti-Phospho-IKB alpha (S32) Antibody [ST53-05]?

The catalog number for Anti-Phospho-IKB alpha (S32) Antibody [ST53-05] is ET1609-78TR.

What sizes are available for Anti-Phospho-IKB alpha (S32) Antibody [ST53-05]?

Anti-Phospho-IKB alpha (S32) Antibody [ST53-05] is available in the following size(s): 20 µL.

Is Anti-Phospho-IKB alpha (S32) Antibody [ST53-05] in stock?

Anti-Phospho-IKB alpha (S32) Antibody [ST53-05] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Anti-Phospho-IKB alpha (S32) Antibody [ST53-05] used for?

On the basis of both functional and structural considerations, members of the IκB family of proteins can be divided into four groups. The first of these groups, IκB-α, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to the

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

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