Anti-HLA-DR Antibody [SC06-78]

About this product

Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II α/β dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (β-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.

Specifications

Catalog number

ET1610-66TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

DR alpha chain antibody DR alpha chain precursor antibody DRA_HUMAN antibody DRB1 antibody DRB4 antibody Histocompatibility antigen HLA DR alpha antibody HLA class II histocompatibility antigen antibody HLA class II histocompatibility antigen DR alpha chain antibody HLA DR1B antibody HLA DR3B antibody HLA DRA antibody HLA DRA1 antibody HLA DRB1 antibody HLA DRB3 antibody HLA DRB4 antibody HLA DRB5 antibody HLA-DRA antibody HLADR4B antibody HLADRA1 antibody HLADRB antibody Major histocompatibility complex class II DR alpha antibody Major histocompatibility complex class II DR beta 1 antibody Major histocompatibility complex class II DR beta 3 antibody Major histocompatibility complex class II DR beta 4 antibody Major histocompatibility complex class II DR beta 5 antibody MGC117330 antibody MHC cell surface glycoprotein antibody MHC class II antigen DRA antibody MHC II antibody MLRW antibody

Abbreviation

HLA-DRA

Swiss Prot

P01903 Human

Cellular Locus

Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus, Endosome membrane, Lysosome membrane.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human HLA-DRA aa 159-195 / 254.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

Daudi cell lysates, Raji cell lysates, Hela, B16F1, human tonsil tissue, human liver tissue, human spleen tissue, Jurkat.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 29 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True