Anti-NLRP3 Antibody [SC06-23]

About this product

NLRP3 is expressed predominantly in macrophages and as a component of the inflammasome, detects products of damaged cells such as extracellular ATP and crystalline uric acid. Activated NLRP3 in turn triggers an immune response. Mutations in the NLRP3 gene are associated with a number of organ specific autoimmune diseases. NLRP3 is a component of the innate immune system that functions as a pattern recognition receptor (PRR) that recognizes pathogen-associated molecular patterns (PAMPs). NLRP3 belongs to the NOD-like receptor (NLR) subfamily of PRRs and NLRP3 together with the adaptor ASC protein PYCARD forms a caspase-1 activating complex known as the NLRP3 inflammasome. NLRP3 in the absence of activating signal is kept in an inactive state complexed with HSP90 and SGT1 in the cytoplasm. NLRP3 inflammasome detects danger signals such as crystalline uric acid and extracellular ATP released by damaged cells. These signals release HSP90 and SGT1 from and recruit ASC protein and caspase-1 to the inflammasome complex. Caspase-1 within the activated NLRP3 inflammasome complex in turn activates the inflammatory cytokine, IL-1β. The NLRP3 inflammasome appears to be activated by changes in intracellular potassium caused by potassium efflux from mechanosensitive ion channels located in the cell membrane

Specifications

Catalog number

ET1610-93TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

AGTAVPRL antibody AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like antibody Angiotensin/vasopressin receptor AII/AVP-like antibody C1orf7 antibody Caterpiller protein 1.1 antibody CIAS 1 antibody CIAS1 antibody CLR1.1 antibody Cold autoinflammatory syndrome 1 antibody Cold autoinflammatory syndrome 1 protein antibody Cryopyrin antibody Familial cold autoinflammatory syndrome antibody FCAS antibody FCU antibody LRR and PYD domains-containing protein 3 antibody Muckle-Wells syndrome antibody MWS antibody NACHT antibody NACHT LRR and PYD containing protein 3 antibody NALP 3 antibody NALP3 antibody NALP3_HUMAN antibody NLRP3 antibody PYPAF 1 antibody PYPAF1 antibody PYRIN containing APAF1 like protein 1 antibody PYRIN-containing APAF1-like protein 1 antibody

Abbreviation

CLR1.1, NLRP3

Swiss Prot

Q96P20 Human; Q8R4B8 Mouse

Cellular Locus

Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human NLRP3 aa 5-161 / 1036.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IP

Positive Control

Human lung tissue lysates, Hela, PMVEC, mouse bladder tissue, mouse spleen tissue, human lung carcinoma tissue, Jurkat, THP-1 cell lysate, RAW264.7 cell lysate.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:1,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:50-1:400 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 118 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True