Anti-ATG5 Antibody [SN73-07]

About this product

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.

Specifications

Catalog number

ET1611-38TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

APG 5 antibody APG 5L antibody APG5 antibody APG5 autophagy 5 like antibody APG5 like antibody APG5-like antibody APG5L antibody Apoptosis specific protein antibody Apoptosis-specific protein antibody ASP antibody ATG 5 antibody Atg5 antibody ATG5 autophagy related 5 homolog antibody ATG5_HUMAN antibody Autophagy protein 5 antibody Autophagy related 5 antibody hAPG5 antibody Homolog of S Cerevisiae autophagy 5 antibody OTTHUMP00000040507 antibody

Abbreviation

ATG5

Swiss Prot

Q9H1Y0 Human; Q99J83 Mouse; Q3MQ06 Rat

Cellular Locus

Cytoplasm, Membrane.

Host

Rabbit

Species Reactivity

Human, Rat, Mouse, Monkey

Immunogen

Synthetic peptide within Human ATG5 aa 1-50 / 275.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Positive Control

NIH/3T3 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa cell lysate, A431 cell lysate, Hela, MCF-7, human lung carcinoma tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 32 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True