Anti-ATF4 Antibody [SD20-92]

About this product

Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promoter and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA. Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family.

Specifications

Catalog number

ET1612-37TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

Activating transcription factor 4 antibody ATF 4 antibody ATF4 antibody ATF4 protein antibody ATF4_HUMAN antibody cAMP-dependent transcription factor ATF-4 antibody cAMP-responsive element-binding protein 2 antibody CREB 2 antibody CREB-2 antibody CREB2 antibody Cyclic AMP dependent transcription factor ATF 4 antibody Cyclic AMP response element binding protein 2 antibody Cyclic AMP-dependent transcription factor ATF-4 antibody Cyclic AMP-responsive element-binding protein 2 antibody DNA binding protein TAXREB67 antibody DNA-binding protein TAXREB67 antibody Tax Responsive Enhancer Element B67 antibody Tax-responsive enhancer element-binding protein 67 antibody TaxREB67 antibody TXREB antibody

Abbreviation

cAMP-dependent transcription factor ATF-4, CREB-2, cAMP-responsive element-binding protein 2, TaxREB67, ATF4

Swiss Prot

P18848 Human; Q06507 Mouse; Q9ES19 Rat

Cellular Locus

Nucleus, Nucleus speckle, Cell membrane, Cytoplasm, Centrosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within Human ATF4 aa 1-220 / 351.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Positive Control

Hela cell lysate, PC-12 cell lysate, HL-60 cell lysate, K562 cell lysate, human lung carcinoma tissue lysate, N2A, human liver carcinoma tissue, human prostate carcinoma tissue, human skin tissue, human breast carcinoma tissue, human stomach carcinoma tissue, human small intestine tissue, human colon carcinoma tissue, Hela, mouse testis tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 39 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True