Anti-LC3B Antibody [JJ090-6]

About this product

Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α) are subunits of both MAP1A and MAP1B. MAP-LC3β, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3β, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3′ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

Specifications

Catalog number

ET1701-65TR

Size / count

20 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Product Name Alternative

ATG8F antibody Autophagy-related protein LC3 B antibody Autophagy-related ubiquitin-like modifier LC3 B antibody LC3B antibody LC3II antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1ALC3 antibody MAP1LC3B a antibody Map1lc3b antibody Microtubule associated protein 1 light chain 3 beta antibody Microtubule-associated protein 1 light chain 3 beta antibody Microtubule-associated proteins 1A/1B light chain 3B antibody MLP3B_HUMAN antibody

Abbreviation

MAP1A/MAP1B LC3 B, MAP1LC3B

Swiss Prot

Q9GZQ8 Human; Q9CQV6 Mouse; Q62625 Rat

Cellular Locus

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within human LC3 B aa 1-20 and aa 80-120.

Isotype

IgG

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IP, IF-Cell

Positive Control

HCT 116 cell lysate, HCT 116 treated with 50μM Chloroquine for 18 hours cell lysate, U-87 MG cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa cells treated with 50μM Chloroquine for 24 hours.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000 ;IP: Use at an assay dependent concentration. ;IF-Cell: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 14/16 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Recombinant Antibody

True