Anti-Phospho-MLKL (S345) Antibody [JM92-37] — image 1 of 2

Product details

Anti-Phospho-MLKL (S345) Antibody [JM92-37]

Catalog number ET1705-51TR

Price for ET1705-51TR

Size / count 20 µL

About this product

Mixed lineage kinase domain like pseudokinase (MLKL) is a protein that in humans is encoded by the MLKL gene. This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Specifications

Catalog number: ET1705-51TR
Size / count: 20 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Product Name Alternative: 9130019I15Rik antibody FLJ34389 antibody hMLKL antibody Mixed lineage kinase domain like antibody Mixed lineage kinase domain like protein antibody Mixed lineage kinase domain like pseudokinase antibody Mixed lineage kinase domain-like protein antibody Mlkl antibody MLKL_HUMAN antibody
Abbreviation: Mlkl
Swiss Prot: Q9D2Y4 Mouse
Cellular Locus: Cell membrane, Cytoplasm, Nucleus.
Host: Rabbit
Species Reactivity: Mouse
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser345 of mouse MLKL aa 314-363 / 472.
Isotype: IgG
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB, IHC-P
Positive Control: L929 treated with 20μM Z-VAD for 3.5 hours then added 100nM SM-164 and 20ng/ml TNF-α for 3 hours cell lysate, mouse spleen tissue, mouse lung tissue, mouse liver tissue, mouse colon tissue.
Concentration: 1 mg/mL
Dilution: WB:1:1,000 ;IHC-P: 1:50-1:100
Purity: Protein A affinity purified.
Form: Liquid
Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 54 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody: True

Frequently Asked Questions

What is the catalog number for Anti-Phospho-MLKL (S345) Antibody [JM92-37]?

The catalog number for Anti-Phospho-MLKL (S345) Antibody [JM92-37] is ET1705-51TR.

What sizes are available for Anti-Phospho-MLKL (S345) Antibody [JM92-37]?

Anti-Phospho-MLKL (S345) Antibody [JM92-37] is available in the following size(s): 20 µL.

Is Anti-Phospho-MLKL (S345) Antibody [JM92-37] in stock?

Anti-Phospho-MLKL (S345) Antibody [JM92-37] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Anti-Phospho-MLKL (S345) Antibody [JM92-37] used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.